The exploration of metals in biology and medicine is a fast-growing research area. Over the past decades, copper, iron and zinc have attracted enormous attentions due to the involvement of metals in aging and neuroscience. These metals can be detected and monitored in vivo by multi-techniques and multi-omics approaches, which further our understanding in metal homeostasis and metals in pathophysiological processes. This workshop aims to share some insightful views and ideas in molecular imaging and biosensing, metals in medicine, antibiotics and drug resistance.